Characterization of an Endolysin Targeting Clostridioides difficile That Affects Spore Outgrowth.

ABSTRACT: Clostridioides difficile is a spore-forming enteric pathogen causing life-threatening diarrhoea and colitis. Microbial disruption caused by antibiotics has been linked with susceptibility to, and transmission and relapse of, C. difficile infection. Therefore, there is an urgent need for novel therapeutics that are effective in preventing C. difficile growth, spore germination, and outgrowth. In recent years bacteriophage-derived endolysins and their derivatives show promise as a novel class of antibacterial agents. In this study, we recombinantly expressed and characterized a cell wall hydrolase (CWH) lysin from C. difficile phage, phiMMP01. The full-length CWH displayed lytic activity against selected C. difficile strains. However, removing the N-termina

SUBMITTER: Mondal SI 

PROVIDER: S-EPMC8199566 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature