SPECT/CT Imaging, Biodistribution and Radiation Dosimetry of a ¹⁷⁷Lu-DOTA-Integrin αvβ6 Cystine Knot Peptide in a Pancreatic Cancer Xenograft Model.

ABSTRACT:

Introduction

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive malignant neoplasms, as many cases go undetected until they reach an advanced stage. Integrin αvβ6 is a cell surface receptor overexpressed in PDAC. Consequently, it may serve as a target for the development of probes for imaging diagnosis and radioligand therapy. Engineered cystine knottin peptides specific for integrin αvβ6 have recently been developed showing high affinity and stability. This study aimed to evaluate an integrin αvβ6-specific knottin molecular probe containing the therapeutic radionuclide ¹⁷⁷Lu for targeting of PDAC.

Methods

The expression of integrin αvβ6 in PDAC cell lines BxPC-3 and Capan-2 was analyzed using RT-qPCR and immunofluorescence. In vitro competition

SUBMITTER: Sachindra S 

PROVIDER: S-EPMC8200818 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature