Landscape and selection of vaccine epitopes in SARS-CoV-2.

ABSTRACT:

Background

Early in the pandemic, we designed a SARS-CoV-2 peptide vaccine containing epitope regions optimized for concurrent B cell, CD4⁺ T cell, and CD8⁺ T cell stimulation. The rationale for this design was to drive both humoral and cellular immunity with high specificity while avoiding undesired effects such as antibody-dependent enhancement (ADE).

Methods

We explored the set of computationally predicted SARS-CoV-2 HLA-I and HLA-II ligands, examining protein source, concurrent human/murine coverage, and population coverage. Beyond MHC affinity, T cell vaccine candidates were further refined by predicted immunogenicity, sequence conservation, source protein abundance, and coverage of high frequency HLA alleles. B cell epitope regions were chosen fro

SUBMITTER: Smith CC 

PROVIDER: S-EPMC8201469 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature