RUNX2 Phosphorylation by Tyrosine Kinase ABL Promotes Breast Cancer Invasion.

ABSTRACT: Activity of transcription factors is normally regulated through interaction with other transcription factors, chromatin remodeling proteins and transcriptional co-activators. In distinction to these well-established transcriptional controls of gene expression, we have uncovered a unique activation model of transcription factors between tyrosine kinase ABL and RUNX2, an osteoblastic master transcription factor, for cancer invasion. We show that ABL directly binds to, phosphorylates, and activates RUNX2 through its SH2 domain in a kinase activity-dependent manner and that the complex formation of these proteins is required for expression of its target gene MMP13. Additionally, we show that the RUNX2 transcriptional activity is dependent on the number of its tyrosine residues that are phospho

SUBMITTER: He F 

PROVIDER: S-EPMC8201617 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature