Electric signals counterbalanced posterior vs anterior PTEN signaling in directed migration of Dictyostelium.

ABSTRACT:

Background

Cells show directed migration response to electric signals, namely electrotaxis or galvanotaxis. PI3K and PTEN jointly play counterbalancing roles in this event via a bilateral regulation of PIP3 signaling. PI3K has been proved essential in anterior signaling of electrotaxing cells, whilst the role of PTEN remains elusive.

Methods

Dictyostelium cells with different genetic backgrounds were treated with direct current electric signals to investigate the genetic regulation of electrotaxis.

Results

We demonstrated that electric signals promoted PTEN phosphatase activity and asymmetrical translocation to the posterior plasma membrane of the electrotaxing cells. Electric stimulation produced a similar but delayed rear redistribution of myosin II, immediately bef

SUBMITTER: Song B 

PROVIDER: S-EPMC8201722 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature