High-throughput developability assays enable library-scale identification of producible protein scaffold variants.

ABSTRACT: Proteins require high developability-quantified by expression, solubility, and stability-for robust utility as therapeutics, diagnostics, and in other biotechnological applications. Measuring traditional developability metrics is low throughput in nature, often slowing the developmental pipeline. We evaluated the ability of 10 variations of three high-throughput developability assays to predict the bacterial recombinant expression of paratope variants of the protein scaffold Gp2. Enabled by a phenotype/genotype linkage, assay performance for 10⁵ variants was calculated via deep sequencing of populations sorted by proxied developability. We identified the most informative assay combination via cross-validation accuracy and correlation feature selection and demonstrated the abilit

SUBMITTER: Golinski AW 

PROVIDER: S-EPMC8201827 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature