Histamine H₃ Receptor Signaling Regulates the NLRP3 Inflammasome Activation in C2C12 Myocyte During Myogenic Differentiation.

ABSTRACT: NLRP3 inflammasome has been implicated in impaired post-injury muscle healing and in muscle atrophy. Histamine receptors play an important role in inflammation, but the role of histamine H₃ receptor (H₃R) in myocyte regeneration and in the regulation of NLRP3 inflammasome is not known. We studied the effects of H₃R signaling on C2C12 myocyte viability, apoptosis, and tumor necrosis factor alpha (TNFα)-induced NLRP3 inflammasome activation during striated myogenic differentiation at three time points (days 0, 3, and 6). Expression of Nlrp3, interleukin-1β (IL-1β), and myogenesis markers were determined. TNFα reduced overall viability of C2C12 cells, and exposure to TNFα induced apoptosis of cells at D6. Activation of H₃R had no effect on vi

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8202077 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature