Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment.

ABSTRACT: Optogenetic protein dimerization systems are powerful tools to investigate the biochemical networks that cells use to make decisions and coordinate their activities. These tools, including the improved Light-Inducible Dimer (iLID) system, offer the ability to selectively recruit components to subcellular locations, such as micron-scale regions of the plasma membrane. In this way, the role of individual proteins within signaling networks can be examined with high spatiotemporal resolution. Currently, consistent recruitment is limited by heterogeneous optogenetic component expression, and spatial precision is diminished by protein diffusion, especially over long time scales. Here, we address these challenges within the iLID system with alternative membrane anchoring domains and fusion config

SUBMITTER: Natwick DE 

PROVIDER: S-EPMC8202217 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature