ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance.
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ABSTRACT:
SUBMITTER: Lismer A
PROVIDER: S-EPMC8202352 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Publications
ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance.
Lismer Ariane A Lambrot Romain R Lafleur Christine C Dumeaux Vanessa V Kimmins Sarah S
STAR protocols 20210611 2
In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implantation embryo chromatin and gene expression. We place emphasis on how to obtain high-quality and quantifiable data sets in sperm and embryos, as ...[more]
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