Virus-induced gene silencing as a tool for functional studies in Cleome violacea.

ABSTRACT:

Premise

Cleomaceae is emerging as a promising family to investigate a wide range of phenomena, such as C₄ photosynthesis and floral diversity. However, functional techniques are lacking for elucidating this diversity. Herein, we establish virus-induced gene silencing (VIGS) as a method of generating functional data for Cleome violacea, bolstering Cleomaceae as a model system.

Methods

We leveraged the sister relationship of Cleomaceae and Brassicaceae by using constructs readily available for Arabidopsis thaliana to provide initial information about the feasibility of VIGS in C. violacea. We then developed endogenous constructs to optimize VIGS efficiency and viability for fruit development.

Results

PHYTOENE DESATURASE was

SUBMITTER: Carey S 

PROVIDER: S-EPMC8202831 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature