E2F2 inhibition induces autophagy via the PI3K/Akt/mTOR pathway in gastric cancer.

ABSTRACT:

Background

E2F2 is a member of the E2F transcription factor family and has important but not fully understood biological functions in cancers. The biological role of E2F2 in gastric cancer (GC) also remains unclear.

Methods

We examined the expression levels of E2F2 in GC using publicly available datasets such as TIMER, Oncomine, GEPIA, UALCAN, etc., and in our patient cohort, using quantitative real-time PCR, western blotting, and immunohistochemistry. We further investigated the effects of E2F2 on phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling, autophagy, and the migration and invasion of GC cells by the wound healing assay, Transwell assay and transmission electron microscopy.

Results

E2F2 was highly expressed in both GC tiss

SUBMITTER: Li H 

PROVIDER: S-EPMC8202834 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature