Marker Genes Change of Synovial Fibroblasts in Rheumatoid Arthritis Patients.

ABSTRACT:

Background

Rheumatoid arthritis (RA) is a chronic condition that manifests as inflammation of synovial joints, leading to joint destruction and deformity.

Methods

We identified single-cell RNA-seq data of synovial fibroblasts from RA and osteoarthritis (OA) patients in GSE109449 dataset. RA- and OA-specific cellular subpopulations were identified, and enrichment analysis was performed. Further, key genes for RA and OA were obtained by combined analysis with differentially expressed genes (DEGs) between RA and OA in GSE56409 dataset. The diagnostic role of key genes for RA was predicted using receiver operating characteristic (ROC) curve. Finally, we identified differences in immune cell infiltration between RA and OA patients, and utilized flow cytometry, qRT-PCR, and Wester

SUBMITTER: Liao L 

PROVIDER: S-EPMC8203351 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature