Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation.

ABSTRACT: Intracellular delivery of cargo molecules such as membrane-impermeable proteins or drugs is crucial for cell treatment in biological and medical applications. Recently, microfluidic mechanoporation techniques have enabled transfection of previously inaccessible cells. These techniques create transient pores in the cell membrane by shear-induced or constriction contact-based rapid cell deformation. However, cells deform and recover differently from a given extent of shear stress or compression and it is unclear how the underlying mechanical properties affect the delivery efficiency of molecules into cells. In this study, we identify cell elasticity as a key mechanical determinant of delivery efficiency leading to the development of "progressive mechanoporation" (PM), a novel mechanoporation

SUBMITTER: Uvizl A 

PROVIDER: S-EPMC8204113 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature