Pathogen identification of Gentiana macrophylla root-knot nematode disease in Yulong, China.

ABSTRACT: In September 2020, samples of galled roots with rhizosphere soil were collected from declining Gentiana macrophylla in Yulong County, China. The pathogenic nematodes were identified by observing morphological characteristics of females, second-stage juveniles and perineal pattern, sequence alignments, and specific amplification of sequence characterized amplified region (SCAR). The results showed that the perineal pattern of this nematode was round or oval, the dorsal arch was moderately high or low, one side or both of the lateral field extended to form a wing shape, the tail region had punctations, and the morphological characteristics and morphometric values of second-stage juveniles and females were similar to those of Meloidogyne hapla. The ITS region fragment of this nematode were hi

SUBMITTER: Wu W 

PROVIDER: S-EPMC8204197 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature