Sensing mechanisms of iron-sulfur cluster regulatory proteins elucidated using native mass spectrometry.

ABSTRACT: The ability to sense and respond to various key environmental cues is important for the survival and adaptability of many bacteria, including pathogens. The particular sensitivity of iron-sulfur (Fe-S) clusters is exploited in nature, such that multiple sensor-regulator proteins, which coordinate the detection of analytes with a (in many cases) global transcriptional response, are Fe-S cluster proteins. The fragility and sensitivity of these Fe-S clusters make studying such proteins difficult, and gaining insight of what they sense, and how they sense it and transduce the signal to affect transcription, is a major challenge. While mass spectrometry is very widely used in biological research, it is normally employed under denaturing conditions where non-covalently attached cofactors are los

SUBMITTER: Crack JC 

PROVIDER: S-EPMC8204329 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature