Residence time analysis of RNA polymerase transcription dynamics: A Bayesian sticky HMM approach.

ABSTRACT: The time spent by a single RNA polymerase (RNAP) at specific locations along the DNA, termed "residence time," reports on the initiation, elongation, and termination stages of transcription. At the single-molecule level, this information can be obtained from dual ultrastable optical trapping experiments, revealing a transcriptional elongation of RNAP interspersed with residence times of variable duration. Successfully discriminating between long and short residence times was used by previous approaches to learn about RNAP's transcription elongation dynamics. Here, we propose an approach based on the Bayesian sticky hidden Markov model that treats all residence times for an Escherichia coli RNAP on an equal footing without a priori discriminating between long and short residence times. Furt

SUBMITTER: Kilic Z 

PROVIDER: S-EPMC8204346 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature