Transition From PCR-Ribotyping to Whole Genome Sequencing Based Typing of Clostridioides difficile.

ABSTRACT: Clostridioides difficile causes nosocomial outbreaks which can lead to severe and even life-threatening colitis. Rapid molecular diagnostic tests allow the identification of toxin-producing, potentially hypervirulent strains, which is critical for patient management and infection control. PCR-ribotyping has been used for decades as the reference standard to investigate transmission in suspected outbreaks. However, the introduction of whole genome sequencing (WGS) for molecular epidemiology provides a realistic alternative to PCR-ribotyping. In this transition phase it is crucial to understand the strengths and weaknesses of the two technologies, and to assess their correlation. We aimed to investigate ribotype prediction from WGS data, and options for analysis at different levels of

SUBMITTER: Seth-Smith HMB 

PROVIDER: S-EPMC8204696 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature