Metagenomic Next-Generation Sequencing for Pathogenic Diagnosis and Antibiotic Management of Severe Community-Acquired Pneumonia in Immunocompromised Adults.

ABSTRACT:

Background

Metagenomic next-generation sequencing (mNGS) is a promising technique for pathogens diagnosis. However, application of mNGS in immunocompromised adults with severe community-acquired pneumonia (SCAP) is relatively limited.

Methods

We retrospectively reviewed 23 immunocompromised and 21 immunocompetent SCAP patients with mNGS detection from April 2019 to December 2019. The performances of pathogenic diagnosis and subsequently antibiotic adjustment in immunocompromised SCAP patients were compared to immunocompetent SCAP patients. The defined by days of therapy (DOT) method was used for estimate daily antibiotic use.

Results

There was a significant difference in the diagnostic positivity rate between mNGS and conventional test in both groups (P<0.001).

SUBMITTER: Sun T 

PROVIDER: S-EPMC8204719 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature