Tailored biosynthesis of gibberellin plant hormones in yeast.

ABSTRACT: The application of small amounts of natural plant growth hormones, such as gibberellins (GAs), can increase the productivity and quality of many vegetable and fruit crops. However, gibberellin growth hormones usage is limited by the high cost of their production, which is currently based on fermentation of a natural fungal producer Fusarium fujikuroi that produces a mix of several GAs. We explored the potential of the oleaginous yeast Yarrowia lipolytica to produce specific profiles of GAs. Firstly, the production of the GA-precursor ent-kaurenoic acid (KA) at 3.75 mg/L was achieved by expression of biosynthetic enzymes from the plant Arabidopsis thaliana and upregulation of the mevalonate (MVA) pathway. We then built a GA₄-producing strain by extending the GA-biosynthetic pathw

SUBMITTER: Kildegaard KR 

PROVIDER: S-EPMC8205117 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature