Sperm parameters and mitochondrial DNA sequence variants among patients at a fertility clinic in Ghana.

ABSTRACT:

Purpose

The present study sought to investigate the common abnormalities and mtDNA mutations in the sperm of Ghanaian men attending the fertility Clinic at the Korle-Bu Teaching Hospital (KBTH). The study therefore provides a baseline data mtDNA mutations in a cross-section of Ghanaian men on referral to the fertility clinic at the KBTH.

Materials and methods

The semen of 55 men attending the fertility clinic were collected from the Urology and the Obstetrics and Gynaecology Departments of the KBTH. Demographic and clinical data were also collected using questionnaires. Semen analyses were performed and were followed by amplification and purification of mtDNA from total DNA extracted from the semen. Sequencing of the mtDNA amplicons was performed using the next generation se

SUBMITTER: Dzudzor B 

PROVIDER: S-EPMC8205148 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature