PFKFB3-dependent glucose metabolism regulates 3T3-L1 adipocyte development.

ABSTRACT: Proliferation and differentiation of preadipocytes, and other cell types, is accompanied by an increase in glucose uptake. Previous work showed that a pulse of high glucose was required during the first 3 days of differentiation in vitro, but was not required after that. The specific glucose metabolism pathways required for adipocyte differentiation are unknown. Herein, we used 3T3-L1 adipocytes as a model system to study glucose metabolism and expansion of the adipocyte metabolome during the first 3 days of differentiation. Our primary outcome measures were GLUT4 and adiponectin, key proteins associated with healthy adipocytes. Using complete media with 0 or 5 mM glucose, we distinguished between developmental features that were dependent on the differentiation cocktail of dexamethasone,

SUBMITTER: Griesel BA 

PROVIDER: S-EPMC8205188 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature