[¹⁸F]PARPi Imaging Is Not Affected by HPV Status In Vitro.

ABSTRACT:

Background

Human papillomavirus- (HPV-) associated oropharyngeal squamous cell carcinomas (OPSCCs) are clinically and pathologically distinct from HPV-negative tumors. Here, we explore whether HPV affects functional biomarkers, including γH2AX, RAD51, and PARP1. Moreover, the role of [¹⁸F]PARPi as a broadly applicable imaging tool for head and neck carcinomas is investigated.

Methods

HPV-positive and HPV-negative cell lines were used to evaluate the γH2AX, RAD51, and PARP1 expression with immunoblotting and immunofluorescence. Effects of external beam ionizing radiation were investigated in vitro, and survival was investigated via colony-formation assay. [¹⁸F]PARPi uptake experiments were performed on HPV-negative and HPV-positive c

SUBMITTER: Guru N 

PROVIDER: S-EPMC8205605 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature