Autophagy protects against cerebral ischemic reperfusion injury by inhibiting neuroinflammation.

ABSTRACT:

Objective

To examine the effect of autophagy on cerebral damage caused by different models and test the hypothesis that its protection mechanism acts via inhibiting expression of neuroinflammatory mediators.

Methods

Autophagy was induced by rapamycin treatment. Cerebral damage was induced using models of IL-6 treatment, oxygen glucose deprivation/reoxygenation (OGD/R) in vitro, and middle cerebral artery occlusion (MCAO) in vivo. The effect and mechanism of autophagy was examined and assessed in terms of cell viability, infarction size in brain tissue, neurological score, production of inflammatory mediators IL-1β and IL-6, transcription and protein expression of autophagy markers beclin-1 and LC-3II in different experimental groups.

Results

Autophagy tr

SUBMITTER: Zha H 

PROVIDER: S-EPMC8205746 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature