A Robust and Rapid Candidate Gene Mapping Pipeline Based on M2 Populations.

ABSTRACT: The whole-genome sequencing-based bulked segregant analysis (WGS-BSA) has facilitated the mapping candidate causal variations for cloning target plant genes. Here, we report an improved WGS-BSA method termed as M2-seq to expedite the mapping candidate mutant loci by studying just M₂ generation. It is an efficient mutant gene mapping tool, rapid, and comparable to the previously reported approaches, such as Mutmap and Mutmap+ that require studying M₃ or advanced selfed generations. In M2-seq, background variations among the M₂ populations can be removed efficiently without knowledge of the variations of the wild-type progenitor plant. Furthermore, the use of absolute delta single-nucleotide polymorphism (SNP) index values can effectively remove the backgroun

SUBMITTER: Zhou H 

PROVIDER: S-EPMC8207192 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature