Rapid identification of mutations caused by fast neutron bombardment in Medicago truncatula.

ABSTRACT:

Background

Fast neutron bombardment (FNB) is a very effective approach for mutagenesis and has been widely used in generating mutant libraries in many plant species. The main type of mutations of FNB mutants are deletions of DNA fragments ranging from few base pairs to several hundred kilobases, thus usually leading to the null mutation of genes. Despite its efficiency in mutagenesis, identification of the mutation sites is still challenging in many species. The traditional strategy of positional cloning is very effective in identifying the mutation but time-consuming. With the availability of genome sequences, the array-based comparative genomic hybridization (CGH) method has been developed to detect the mutation sites by comparing the signal intensities of probes between wild-typ

SUBMITTER: Du H 

PROVIDER: S-EPMC8207604 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature