Two Fusarium copper radical oxidases with high activity on aryl alcohols.

ABSTRACT:

Background

Biomass valorization has been suggested as a sustainable alternative to petroleum-based energy and commodities. In this context, the copper radical oxidases (CROs) from Auxiliary Activity Family 5/Subfamily 2 (AA5_2) are attractive biocatalysts for the selective oxidation of primary alcohols to aldehydes. Originally defined by the archetypal galactose 6-oxidase from Fusarium graminearum, fungal AA5_2 members have recently been shown to comprise a wide range of specificities for aromatic, aliphatic and furan-based alcohols. This suggests a broader substrate scope of native CROs for applications. However, only 10% of the annotated AA5_2 members have been characterized to date.

Results

Here, we define two homologues from the filamentous fungi Fusarium graminearum and

SUBMITTER: Cleveland M 

PROVIDER: S-EPMC8207647 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature