Changing the Wound: Covalent Immobilization of the Epidermal Growth Factor.

ABSTRACT: Re-epithelialization of wounds is a critical element of wound closure. Growth factors have been used in combination with conventional wound management to promote closure, but the method of delivery has been limited to the topical application of ointment formulations. Cytoactive factors delivered in this way have short resident times in wounds and have met with limited success. Here, we demonstrate that methods used to covalently immobilize proteins on synthetic materials can be extended to immobilize cytoactive factors such as the epidermal growth factor (EGF) onto the wound beds of genetically diabetic mice that exhibit impaired healing. Full-thickness splinted excisional wounds were created in diabetic (db/db) mice with a well-defined silicone splint to limit wound contracture. Wound sur

SUBMITTER: Raghunathan V 

PROVIDER: S-EPMC8207651 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature