Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.

ABSTRACT:

Background

Glioma is one of the most common central nervous system malignant tumors, accounting for 45~60% of adult intracranial tumors. However, the clinical treatment of glioma is limited. It is of great significance to seek new therapeutic methods for glioma via gene therapy.

Methods

Long non-coding RNA (lncRNA) SNHG16 expression level was measured by microarray and qRT-PCR assay; ISH was used to identify the location of SNHG16. Cancer stem cells (CSCs) were separated from glioma tissues and identified using immunofluorescence. Exosomes were isolated from CSCs and cancer cells and identified by TEM and western blot. MTT, wound healing, transwell, and colony formation assay were performed to explore the role of SNHG16 or si-SNHG16 from CSCs on progression of glioma cells.

SUBMITTER: Zhang R 

PROVIDER: S-EPMC8207674 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature