IL-33/ST2 axis deficiency exacerbates neutrophil-dominant allergic airway inflammation.

ABSTRACT:

Objective

The IL-33/ST2 axis has been extensively investigated in type 2 eosinophilic inflammation. Here, we aimed to investigate the role of the IL-33/ST2 axis in neutrophil-dominant allergic airway inflammation.

Methods

House-dust mite (HDM) extract and lipopolysaccharide (LPS) were administered to establish a murine model of neutrophil-dominant allergic airway inflammation. The formation of neutrophilic extracellular traps (NETs) in the lung tissues was demonstrated by immunofluorescence imaging. Mature IL-33 in bronchoalveolar lavage fluid (BALF) was detected by Western blotting. The neutrophilic chemokine KC produced by bone marrow-derived macrophages (BMDMs) or primary alveolar epithelial cells was measured with a commercial ELISA kit.

Results

In the present stu

SUBMITTER: Ma Q 

PROVIDER: S-EPMC8207976 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature