Sigma factor dependent translational activation in Bacillus subtilis.

ABSTRACT: Sigma factors are an important class of bacterial transcription factors that lend specificity to RNA polymerases by binding to distinct promoter elements for genes in their regulons. Here we show that activation of the general stress sigma factor, σ^B, in Bacillus subtilis paradoxically leads to dramatic induction of translation for a subset of its regulon genes. These genes are translationally repressed when transcribed by the housekeeping sigma factor, σ^A, owing to extended RNA secondary structures as determined in vivo using DMS-MaPseq. Transcription from σ^B-dependent promoters ablates the secondary structures and activates translation, leading to dual induction. Translation efficiencies between σ^B- and σ^A-dependent RNA

SUBMITTER: McCormick DM 

PROVIDER: S-EPMC8208050 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature