A Mycoplasma gallisepticum Glycerol ABC Transporter Involved in Pathogenicity.

ABSTRACT: MalF has been shown to be required for virulence in the important avian pathogen Mycoplasma gallisepticum To characterize the function of MalF, predicted to be part of a putative ABC transporter, we compared metabolite profiles of a mutant with a transposon inserted in malF (MalF-deficient ST mutant 04-1; ΔmalF) with those of wild-type bacteria using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Of the substrates likely to be transported by an ABC transport system, glycerol was detected at significantly lower abundance in the ΔmalF mutant, compared to the wild type. Stable isotope labeling using [U-¹³C]glycerol and reverse transcription-quantitative PCR analysis indicated that MalF was responsible for the import of

SUBMITTER: Mahdizadeh S 

PROVIDER: S-EPMC8208142 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature