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A minimal hybridization chain reaction (HCR) system using peptide nucleic acids.

ABSTRACT: The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of γ-peg-modified PNA was found to be beneficial.

SUBMITTER: Kim KT

PROVIDER: S-EPMC8208298 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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A minimal hybridization chain reaction (HCR) system using peptide nucleic acids.

Kim Ki Tae KT Angerani Simona S Winssinger Nicolas N

Chemical science 20210506 23

The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on ...[more]

PMID: 34194712

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