Genome-wide analysis in Escherichia coli unravels a high level of genetic homoplasy associated with cefotaxime resistance.

ABSTRACT: Cefotaxime (CTX) is a third-generation cephalosporin (3GC) commonly used to treat infections caused by Escherichia coli. Two genetic mechanisms have been associated with 3GC resistance in E. coli. The first is the conjugative transfer of a plasmid harbouring antibiotic-resistance genes. The second is the introduction of mutations in the promoter region of the ampC β-lactamase gene that cause chromosome-encoded β-lactamase hyperproduction. A wide variety of promoter mutations related to AmpC hyperproduction have been described. However, their link to CTX resistance has not been reported. We recultured 172 cefoxitin-resistant E. coli isolates with known CTX minimum inhibitory concentrations and performed genome-wide analysis of homoplastic mutations associated wit

SUBMITTER: Coolen JPM 

PROVIDER: S-EPMC8208684 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature