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Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes.

ABSTRACT: During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca²⁺ activity can be performed continuously for 2-3 days and even up to several months. For complete details on the use and execution of this protocol, please refer to Gengatharan et al. (2021).

SUBMITTER: Malvaut S

PROVIDER: S-EPMC8209737 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes.

Malvaut Sarah S Marymonchyk Alina A Gengatharan Archana A Saghatelyan Armen A

STAR protocols 20210612 2

During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca2+ activity can be performed continuously for ...[more]

PMID: 34169290

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