Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay.
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ABSTRACT:
SUBMITTER: Nablo BJ
PROVIDER: S-EPMC8209770 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay.
Nablo Brian J BJ Ahn Jung-Joon JJ Bhadriraju Kiran K Lee Jong Muk JM Reyes Darwin R DR
ACS applied bio materials 20200101 10
Neutral red is a low-cost supravital stain for determining cell viability. The standard protocol relies on a destructive extraction process to release the accumulated dye for endpoint spectrophotometric quantification. We report a non-destructive, live-cell quantification of neutral red uptake using a compact lens-free system. Two light sources indentify the cell perimeter and quantify neutral red uptake. The quantification occurs during staining, thus eliminating the destructive extraction proc ...[more]
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