Successive High-Resolution (H₂O)_n-GCIB and C₆₀-SIMS Imaging Integrates Multi-Omics in Different Cell Types in Breast Cancer Tissue.

ABSTRACT: The temporo-spatial organization of different cells in the tumor microenvironment (TME) is the key to understanding their complex communication networks and the immune landscape that exists within compromised tissues. Multi-omics profiling of single-interacting cells in the native TME is critical for providing further information regarding the reprograming mechanisms leading to immunosuppression and tumor progression. This requires new technologies for biomolecular profiling of phenotypically heterogeneous cells on the same tissue sample. Here, we developed a new methodology for comprehensive lipidomic and metabolomic profiling of individual cells on frozen-hydrated tissue sections using water gas cluster ion beam secondary ion mass spectrometry ((H₂O)_n-GCIB-SI

SUBMITTER: Tian H 

PROVIDER: S-EPMC8209780 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature