Stabilization of an ²¹¹At-Labeled Antibody with Sodium Ascorbate.

ABSTRACT: ²¹¹At, an α-particle emitter, has recently attracted attention for radioimmunotherapy of intractable cancers. However, our sodium dodecyl sulfate polyacrylamide gel electrophoresis and flow cytometry analyses revealed that ²¹¹At-labeled immunoconjugates are easily disrupted. Luminol assay revealed that reactive oxygen species generated from radiolysis of water caused the disruption of ²¹¹At-labeled immunoconjugates. To retain their functions, we explored methods to protect ²¹¹At-immunoconjugates from oxidation and enhance their stability. Among several other reducing agents, sodium ascorbate most safely and successfully protected ²¹¹At-labeled trastuzumab from oxidative stress and retained the stability of the ²¹¹At-labeled

SUBMITTER: Manabe S 

PROVIDER: S-EPMC8209801 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature