High prevalence of bla _CTX-M-15 and nosocomial transmission of hypervirulent epidemic clones of Klebsiella pneumoniae at a tertiary hospital in Ethiopia.

ABSTRACT:

Background

Genomic epidemiology of antibiotic resistance is not sufficiently studied in low-income countries.

Objectives

To determine prevalence of ESBL production, and resistome and virulome profiles, of Klebsiella pneumoniae isolated at Jimma Medical Center, Ethiopia.

Methods

Strains isolated from patients with suspected infections between June and November 2016 were characterized by MALDI-TOF for species identification and disc diffusion for antimicrobial susceptibility testing. All K. pneumoniae isolates were characterized by double disc diffusion for ESBL production and all ESBL-producing strains (ESBL-KP) were subjected to WGS on the Illumina (HiSeq 2500) platform. DNA was extracted by automated systems (MagNA Pure 96). Genome assembly was performe

SUBMITTER: Sewunet T 

PROVIDER: S-EPMC8210115 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature