Ribosomal RNA 2'O-methylation as a novel layer of inter-tumour heterogeneity in breast cancer.

ABSTRACT: Recent epitranscriptomics studies unravelled that ribosomal RNA (rRNA) 2'O-methylation is an additional layer of gene expression regulation highlighting the ribosome as a novel actor of translation control. However, this major finding lies on evidences coming mainly, if not exclusively, from cellular models. Using the innovative next-generation RiboMeth-seq technology, we established the first rRNA 2'O-methylation landscape in 195 primary human breast tumours. We uncovered the existence of compulsory/stable sites, which show limited inter-patient variability in their 2'O-methylation level, which map on functionally important sites of the human ribosome structure and which are surrounded by variable sites found from the second nucleotide layers. Our data demonstrate that some positions with

SUBMITTER: Marcel V 

PROVIDER: S-EPMC8210124 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature