Characterization of the MurT/GatD complex in <i>Mycobacterium tuberculosis</i> towards validating a novel anti-tubercular drug target.

ABSTRACT:

Objectives

Identification and validation of novel therapeutic targets is imperative to tackle the rise of drug resistance in tuberculosis. An essential Mur ligase-like gene (Rv3712), expected to be involved in cell-wall peptidoglycan (PG) biogenesis and conserved across mycobacteria, including the genetically depleted Mycobacterium leprae, was the primary focus of this study.

Methods

Biochemical analysis of Rv3712 was performed using inorganic phosphate release assays. The operon structure was identified using reverse-transcriptase PCR and a transcription/translation fusion vector. In vivo mycobacterial protein fragment complementation assays helped generate the interactome.

Results

Rv3712 was found to be an ATPase. Characterization of its operon reveale

SUBMITTER: Maitra A 

PROVIDER: S-EPMC8210147 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature