Rapid, Sample-to-Answer Host Gene Expression Test to Diagnose Viral Infection.

ABSTRACT:

Objective

Distinguishing bacterial, viral, or other etiologies of acute illness is diagnostically challenging with significant implications for appropriate antimicrobial use. Host gene expression offers a promising approach, although no clinically useful test has been developed yet to accomplish this. Here, Qvella's FAST HR (Richmond Hill, Ontario, Canada) process was developed to quantify previously identified host gene expression signatures in whole blood in <45 minutes.

Method

Whole blood was collected from 128 human subjects (mean age 47, range 18-88) with clinically adjudicated, microbiologically confirmed viral infection, bacterial infection, noninfectious illness, or healthy controls. Stabilized mRNA was released from cleaned and stabilized RNA-surfactant complexes us

SUBMITTER: Tsalik EL 

PROVIDER: S-EPMC8210837 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature