Massively-Parallelized, Deterministic Mechanoporation for Intracellular Delivery.

ABSTRACT: Microfluidic intracellular delivery approaches based on plasma membrane poration have shown promise for addressing the limitations of conventional cellular engineering techniques in a wide range of applications in biology and medicine. However, the inherent stochasticity of the poration process in many of these approaches often results in a trade-off between delivery efficiency and cellular viability, thus potentially limiting their utility. Herein, we present a novel microfluidic device concept that mitigates this trade-off by providing opportunity for deterministic mechanoporation (DMP) of cells en masse. This is achieved by the impingement of each cell upon a single needle-like penetrator during aspiration-based capture, followed by diffusive influx of exogenous cargo through the result

SUBMITTER: Dixit HG 

PROVIDER: S-EPMC8210888 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature