A Semiautomated Paramagnetic Bead-Based Platform for Isobaric Tag Sample Preparation.

ABSTRACT: The development of streamlined and high-throughput sample processing workflows is important for capitalizing on emerging advances and innovations in mass spectrometry-based applications. While the adaptation of new technologies and improved methodologies is fast paced, automation of upstream sample processing often lags. Here we have developed and implemented a semiautomated paramagnetic bead-based platform for isobaric tag sample preparation. We benchmarked the robot-assisted platform by comparing the protein abundance profiles of six common parental laboratory yeast strains in triplicate TMTpro16-plex experiments against an identical set of experiments in which the samples were manually processed. Both sets of experiments quantified similar numbers of proteins and peptides with good repr

SUBMITTER: Liu X 

PROVIDER: S-EPMC8210952 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature