Composition and Biophysical Properties of the Sorting Platform Pods in the <i>Shigella</i> Type III Secretion System.

ABSTRACT: Shigella flexneri, causative agent of bacillary dysentery (shigellosis), uses a type III secretion system (T3SS) as its primary virulence factor. The T3SS injectisome delivers effector proteins into host cells to promote entry and create an important intracellular niche. The injectisome's cytoplasmic sorting platform (SP) is a critical assembly that contributes to substrate selection and energizing secretion. The SP consists of oligomeric Spa33 "pods" that associate with the basal body via MxiK and connect to the Spa47 ATPase via MxiN. The pods contain heterotrimers of Spa33 with one full-length copy associated with two copies of a C-terminal domain (Spa33^C). The structure of Spa33^C is known, but the precise makeup and structure of the pods in

SUBMITTER: Tachiyama S 

PROVIDER: S-EPMC8211105 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature