Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia.

ABSTRACT: Classification of clinical symptoms and diagnostic microbiology are essential to effectively employ antimicrobial therapy for lower respiratory tract infections (LRTIs) in a timely manner. Empirical antibiotic treatment without microbial identification hinders the selective use of narrow-spectrum antibiotics and effective patient treatment. Thus, the development of rapid and accurate diagnostic procedures that can be readily adopted by the clinic is necessary to minimize non-essential or excessive use of antibiotics and accelerate patient recovery from LRTI-induced damage. We developed and validated a multiplex real-time polymerase chain reaction (mRT-PCR) assay with good analytical performance and high specificity to simultaneously detect four bacterial pathogens causing pneumonia: Klebsi

SUBMITTER: Lim HJ 

PROVIDER: S-EPMC8211157 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature