Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems.

ABSTRACT:

Background

Whilst traditional strategies to increase transfection efficiency of non-viral systems aimed at modifying the vector or the polyplexes/lipoplexes, biomaterial-mediated gene delivery has recently sparked increased interest. This review aims at discussing biomaterial properties and unravelling underlying mechanisms of action, for biomaterial-mediated gene delivery. DNA internalisation and cytoplasmic transport are initially discussed. DNA immobilisation, encapsulation and surface-mediated gene delivery (SMD), the role of extracellular matrix (ECM) and topographical cues, biomaterial stiffness and mechanical stimulation are finally outlined.

Main text

Endocytic pathways and mechanisms to escape the lysosomal network are highly variable. They depend on cell and DNA co

SUBMITTER: Graceffa V 

PROVIDER: S-EPMC8211807 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature