Reprogramming signal transduction through a designer receptor tyrosine kinase.

ABSTRACT: Controlling signal transduction with artificial designer receptors is a promising approach to realize future medicine for intractable diseases. Although several functional artificial receptors have been reported by domain engineering, more sophisticated engineering within domains has yet to be thoroughly investigated. Here we demonstrate motif-based engineering of a receptor tyrosine kinase for reprogramming signal transduction. We design a scaffold-less tyrosine kinase domain that does not recruit any signal transducers but retains its kinase function. The resultant scaffold-less tyrosine kinase domain is linked to a tyrosine motif that recruits a target signaling molecule upon its phosphorylation. The engineered tyrosine motif-kinase fusion protein is further connected to a small molecul

SUBMITTER: Kongkrongtong T 

PROVIDER: S-EPMC8211861 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature