DNA-Functionalized Metal-Organic Framework Nanoparticles for Intracellular Delivery of Proteins.

ABSTRACT: Due to their large size, charged surfaces, and environmental sensitivity, proteins do not naturally cross cell-membranes in intact form and, therefore, are difficult to deliver for both diagnostic and therapeutic purposes. Based upon the observation that clustered oligonucleotides can naturally engage scavenger receptors that facilitate cellular transfection, nucleic acid-metal organic framework nanoparticle (MOF NP) conjugates have been designed and synthesized from NU-1000 and PCN-222/MOF-545, respectively, and phosphate-terminated oligonucleotides. They have been characterized structurally and with respect to their ability to enter mammalian cells. The MOFs act as protein hosts, and their densely functionalized, oligonucleotide-rich surfaces make them colloidally stable and ensure facil

SUBMITTER: Wang S 

PROVIDER: S-EPMC8212418 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature