Aggregation-induced integrated stress response rejuvenates culture-expanded human mesenchymal stem cells.

ABSTRACT: Protein homeostasis is critical for cellular function, as loss of homeostasis is attributed to aging and the accumulation of unwanted proteins. Human mesenchymal stem cells (MSCs) have shown promising therapeutic potential due to their impressive abilities to secrete inflammatory modulators, angiogenic, and regenerative cytokines. However, there exists the problem of human MSC expansion with compromised therapeutic quality. Duringin vitro expansion, human MSCs are plated on stiff plastics and undergo culture adaptation, which results in aberrant proliferation, shifts in metabolism, and decreased autophagic activity. It has previously been shown that three-dimensional (3D) aggregation can reverse some of these alterations by heightening autophagy and recovering the metabolic state back to a

SUBMITTER: Bijonowski BM 

PROVIDER: S-EPMC8212669 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature