Haem is crucial for medium-dependent metronidazole resistance in clinical isolates of Clostridioides difficile.

ABSTRACT:

Background

Until recently, metronidazole was the first-line treatment for Clostridioides difficile infection and it is still commonly used. Though resistance has been reported due to the plasmid pCD-METRO, this does not explain all cases.

Objectives

To identify factors that contribute to plasmid-independent metronidazole resistance of C. difficile.

Methods

Here, we investigate resistance to metronidazole in a collection of clinical isolates of C. difficile using a combination of antimicrobial susceptibility testing on different solid agar media and WGS of selected isolates.

Results

We find that nearly all isolates demonstrate a haem-dependent increase in the MIC of metronidazole, which in some cases leads to isolates qualifying as resistant (MIC >2 mg/L). Moreo

SUBMITTER: Boekhoud IM 

PROVIDER: S-EPMC8212768 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature